Real Time-qPCR (RT-qPCR) Solutions
United States (USA) Centers for Disease Control and Prevention (CDC) qPCR Diagnostic Kit
RT-PCR primers and probes designed by the US Centers for Disease Control and Prevention (CDC) for the detection of SARS-CoV-2 virus are produced under ISO 13485: 2016 conditions with IDT assurance. . In addition to the primers, the kit includes 5' FAM - ZEN™ / Iowa Black® FQ or 5' FAM / 3' Black Hole Quencher® (BHQ) probes. 2019-nCoV controls are also provided.
Diagnostic Kit According to Charité/Berlin (WHO) Protocol
RT-PCR primers and probes designed by researchers at Charité – Universitätsmedizin Berlin Institute of Virology, German Center for Infection Research, Tib-Molbiol, Erasmus MC, Rotterdam and other institutions for the detection of SARS-CoV-2 are manufactured under ISO 13485: 2016 conditions with IDT assurance. . With the kit, also known as the Berlin Panel, RdRp and E genes are analyzed to detect the positivity of SARS-CoV-2. Kit includes 5' FAM – ZEN™ / Iowa Black® FQ probes in addition to primers.
SARS-CoV-2 qPCR Panel Design
Primers and probes with unmatched reliability and purity for diagnostic tests, the fundamental step in the fight against COVID-19, are delivered quickly according to your design. You can contact us to get support on design and to get more information.
One-step RT-qPCR kits
One Step PrimeScript™ RT-PCR Kit
One Step PrimeScript RT-PCR is a single-stage RT-PCR kit that works with probe capture.
All RT-PCR steps can be performed in a single tube. No need to add reagents during the reaction, minimizing the risk of contamination and simplifying the workflow. The kit can be used to detect small amounts of RNA.
One Step PrimeScript III RT-PCR Kit
Single step RT-qPCR and < 1 hour reaction time.
One Step PrimeScript RT-PCR III RT-qPCR Mix is a 2X RT-qPCR master mix working with a single step probe capture method (5' nuclease method). It contains the heat and inhibitor resistant PrimeScript III RTase reverse transcriptase enzyme, the hot start PCR enzyme Takara Taq HS and an optimized reaction buffer solution.
Thanks to its fast and simple protocol, it allows reverse transcription and qPCR reactions to be performed in the same tube, reducing the risk of contamination.
PrimeDirect Probe RT-qPCR Mix
PrimeDirect Probe RT-qPCR Mix is designed for one-step RT-PCR studies using the probe capture method (5′-nuclease method). Supplied as 2X to allow easy preparation of reaction mixtures. Primary sample (blood, swab, etc.) serum, cell, etc.) provides the opportunity to work with.
Next Generation Sequencing Solutions
You can find our next-generation sequencing solutions below to conduct surveillance work of the SARS-Cov-2 virus and detect its evolution and spread.
Illumina COVIDSeq Test (RUO)
The in vitro diagnostic (IVD) version of this product is FDA-approved for Emergency Use (EUA) for use only in the United States (USA). Suitable for research purposes only (RUO) use in countries outside the USA.
The amplicon-based Illumina COVIDSeq next-generation sequencing panel includes 2019-nCoV primers designed to detect RNA of the SARS-CoV-2 virus in nasopharyngeal, oropharyngeal, and middle turbinate nasal swab samples from individuals with symptoms compatible with COVID-19.COVIDSeq Assay uses a modified version of the validated and publicly available ARTIC multiplex PCR protocol with 98 amplicons designed to amplify SARS-CoV-2 virus-specific sequences. Each sample contains an internal control of 11 human mRNA targets.
Accuracy: 98 sites are targeted in SARS-CoV-2 for high accuracy detection.
Yield: 3072 samples result in 12 hours on the NovaSeq 6000 system. It gives 384 sample results in 12 hours on NextSeq500 / 550 / 550Dx on systems with RUO mode.
Quality Control: Each reaction has an internal control of 11 human mRNA targets.
Flexibility: Can be run on NovaSeq 6000 S4 and SP flow cells and NextSeq 500/550 / 550Dx RUO mode HO flow cell.
Respiratory Virus Oligos Panel
Illumina's Respiratory Virus Oligos Panel includes strains of Influenza A/B, Adenovirus, Rhinovirus, Bocavirus, etc., including SARS-Cov-2 and SARS-Cov strains. It provides high-sensitivity detection and characterization of common respiratory viruses. Oligo probes are effective even in highly mutagenic regions and allow targeting of rapidly evolving RNA viruses. It can be used with the Illumina RNA Prep with Enrichment kit.
Resiratory Virus Oligo Panel ile Tespit Edilen Virüsler
- Human coronavirus 229E
- Human coronavirus NL63
- Human coronavirus OC43
- Human coronavirus HKU1
- SARS-CoV-2
- Human adenovirus B1
- Human adenovirus C2
- Human adenovirus E4
- Human bocavirus 1 (Primate bocaparvovirus 1 isolate st2)
- Human bocavirus 2c PK isolate PK-5510
- Human bocavirus 3
- Human bocavirus 4 NI strain HBoV4-NI-385
- KI polyomavirus Stockholm 60
- WU Polyomavirus
- Human parechovirus type 1 PicoBank/HPeV1/a
- Human parechovirus 6
- Human rhinovirus A89
- Human rhinovirus C (strain 024)
- Human rhinovirus B14
- Human enterovirus C104 strain: AK11
- Human enterovirus C109 isolate NICA08-4327
- Human parainfluenza virus 1
- Human parainfluenza virus 2
- Human parainfluenza virus 3
- Human parainfluenza virus 4a
- Human metapneumovirus (CAN97-83)
- Respiratory syncytial virus (type A)
- Human Respiratory syncytial virus 9320 (type B)
- Influenza A virus (A/Puerto Rico/8/1934(H1N1))
- Influenza A virus (A/Korea/426/1968(H2N2))
- Influenza A virus (A/New York/392/2004(H3N2))
- Influenza A virus (A/goose/Guangdong/1/1996(H5N1))
- Influenza A virus (A/Zhejiang/DTIDZJU01/2013(H7N9))
- Influenza A virus (A/Hong Kong/1073/99(H9N2))
- Influenza A virus (A/Texas/50/2012(H3N2))
- Influenza A virus (A/Michigan/45/2015(H1N1))
- Influenza B virus (B/Lee/1940)
- Influenza B virus (B/Wisconsin/01/2010)
- Influenza B virus (B/Brisbane/60/2008)
- Influenza B virus (B/Colorado/06/2017)
- Influenza B virus (B/Washington/02/2019)
- Human control genes
CleanPlex SARS-CoV-2
The Paragon CleanPlex SARS-CoV-2 kit is a next-generation sequencing panel designed for SARS-CoV-2 virus research and surveillance studies. Using the Multiplex PCR method, the entire genome of the SARS-CoV-2 virus can be sequenced with this kit. With Paragon Genomics CleanPlex technology, the library preparation phase from RNA is completed in 5.5 hours.
Droplet Digital PCR (ddPCR) Solution
SARS-CoV-2 Droplet Digital PCR (ddPCR) Kit
With the ddPCR technology, which carries the detection sensitivity to a much higher point, it is possible to obtain sensitive and highly accurate results even in samples with low viral load.
Compatible with Bio-Rad QX200 and QXDx AutoDG systems, the FDA-approved SARS-CoV-2 ddPCR Kit can perform qualitative detection of SARS-CoV-2 nucleic acid from samples such as nasopharyngeal swab/aspirate, nasal swab/aspirate. It has also been shown that sensitivity as low as 0.26-0.29 copies/ul can be achieved in the detection of N1 and N2 genes. The test is a triplex probe assay and provides the determination of N1, N2 and RP genes in a single tube.
*This kit is for research use only.
Biorad SARS-CoV-2 Standard
The Biorad SARS-C0V-2 standard was produced with synthetic RNA transcripts containing 5 target genes (E, N, Orf1ab, RdRP and S Genes). All genes were quantified by Biorad ddPCR at 200,000 cp/ml. It can be used as reference material for validation of different application processes such as extraction, amplification and detection of coronavirus studies.
Biorad SARS-CoV-2 Negative
The SARS-CoV-2 Negative contains 75,000 copies/ml of genomic DNA formulated in a synthetic matrix.
Reference Materials
IDT 2019-nCoV Plasmid Controls
They are plasmid controls containing the entire nucleocapsid region of 2019-nCoV, SARS, and MERS viruses.
- 2019-nCoV_N_Positive Control: Contains the nucleocapsid N gene region of 2019-nCoV.
- Hs_RPP30 Positive Control: Contains the RPP30 gene found in the human genome.
- MERS-CoV
- SARS-CoV
AccuPlex™ SARS-CoV-2 Verification Panel
This panel is used for the optimization of tests in the design phase. It provides important information about the LOD value of the test, the comparison of different test applications, and the work efficiency of the application team. The product contains positive control material containing the entire SARS-COV-2 viral genome, as well as negative control material targeting the RNase P gene in the human genome.AccuPlex™ SARS-CoV-2
Reference Material
This solution, which can be used for daily performance evaluation in tests, contains positive and negative reference material. It contains the entire SARS-CoV-2 genome.
AccuPlex™ SARS-CoV-2, Flu A/B and RSV Verification Panel
The AccuPlex™ SARS-CoV-2, Flu A/B and RSV Verification Panel is used to detect SARS-CoV-2, flu A/B and respiratory syncytial virus (RSV) with the multiplex test method. It contains positive reference material at three different concentrations as well as negative control material engineered to the human RNase P gene.
